Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples. Key Features and Benefits Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions Fast 26 min cDNA synthesis protocol Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase